Bio-Rad Microplate Manager Software - Manual (Page 56)

Microplate Manager User Guide
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Select the data file containing the external standards and click on Open. The names
of all assays with defined standards within the selected data file will appear in the
Source Assay pulldown menu.
Figure 32. Selecting the source assay for your external standards.
Under the Source Assay pulldown menu, select the assay that contains the desired
external standards. When you are finished, click on OK to close the dialog and
implement your changes.
Notes on External Standards
When the source assay is selected for an external standard, the standards data is
copied from the source file into the new data file. Any subsequent changes to the
standards in the source file will not be updated in the new data file. After external
standards have been copied into a data file, the imported standards in that data file
can now be used as the source for subsequent files.
External standards override internal standards. If the External Standards checkbox is
checked, then the concentrations of the unknowns are calculated using the external
standards, even if internal standards are also defined on the plate. To make
calculations using the internal standards, deselect the External Standards checkbox.
In the Unknown Concentration report, external standards are labeled “XSn,” where
n is an integer beginning at 1.