Bio-Rad Microplate Manager Software - Manual (Page 42)

Microplate Manager User Guide
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Figure 20. Example of a plate template with two different assays defined.
Replicate Groups
A replicate group is a group of wells with the same standard or unknown. For each
replicate group, a mean absorbance value (in the case of endpoint or multiple plate
protocols) or velocity (in the case of kinetic protocols) is calculated, along with the
standard deviation and coefficient of variation for the replicate group. If more than
one blank, positive, or negative control is defined for a template, they are treated like
a replicate group, and the same statistical analyses are performed (mean, standard
deviation, and coefficient of variation).
Assays
Up to 12 assays may be defined on a single plate. These are differentiated by color.
The first assay always appears as the standard background gray. If you define
subsequent assays, they appear as different colors. Independent statistical analysis
and data reduction are performed for each assay.
Assay 2 Assay 1
Unknown
Blank
Positive
control
Negative
control
Undefined
Standard
Replicate
Group 1
Standard