Bio-Rad Microplate Manager Software - Manual (Page 52)

Microplate Manager User Guide
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Figure 28. Edit Unknowns dialog box.
Each defined assay has a tab that is labeled with the name of the assay (default names
are Assay 1, Assay 2, etc.). Dilutions for each assay are entered separately on a form
under each tab. To enter dilutions for Assay 2, click on the Assay 2 tab.
Each unknown is listed in the column at the left. The default dilution is 1.
Click on the Enter Manually button to directly input the dilutions of your unknowns.
Then type in the dilution factor in the appropriate fields in the Dilut. column.
½
The number in the Dilut. column is the dilution factor, not the final
concentration. For example, if the first unknown has a dilution factor of 1 (i.e.,
no dilution), and each unknown is twice as diluted as the previous unknown,
enter 2 for the second unknown, 4 for the third unknown, 8 for the fourth
unknown, etc.
The software can calculate the dilution series for you. Click on the Enter
Automatically button, then click on the button that identifies the least diluted
unknown and enter that dilution and the dilution factor in the appropriate fields.