Bio-Rad Microplate Manager Software - Manual (Page 72)

Microplate Manager User Guide
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Figure 44. Analysis Options in the kinetic protocol dialog box.
The settings in this dialog box will only affect the analysis of your data. They will not
change the raw data that is gathered.
Time and Absorbance Limits
In this dialog, you can specify a time range and/or an absorbance range for your data
points. If time limits and/or absorbance limits are set, then only data points within
these limits will be used to calculate the reaction velocity. This is useful for
excluding an initial lag time in delayed reactions or for excluding the end of a
reaction as it approaches equilibrium.
To set time limits, select the appropriate checkbox(es) and enter a number of seconds
in the appropriate field(s).