Bio-Rad Microplate Manager Software - Manual (Page 45)

Chapter 4. Templates
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Figure 22. Defining an assay with the Assay tool.
When the mouse is released, a new assay will be created, and all the wells in the
assay will have the same color.
You can define up to 12 assays on a single template. When you are done defining
your assay(s), click on the Select button on the toolbar to change your cursor back to
normal functionality.
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When a new template is first displayed, all wells are considered to be part of
Assay 1. The first assay defined with the Assay tool is named Assay 2, the
second assay defined with the Assay tool is named Assay 3 and so forth, and all
remaining wells are considered part of Assay 1. If all wells are eventually
reassigned from Assay 1 to another assay, the assay numbers are not resequenced
and there will not be an Assay 1 on the plate or in the reports.
Assay Info
To rename your assays, annotate them, or change their colors, select Assay Info...
from the ASSAY menu. This will open the Assay Information dialog box.