Luminex 100 User Manual Version 1.7 - Manual (Page 100)

Luminex 100 User Manual Version 1.7 x MAP Technology
8 - 6 PN 89-00002-00-063 Rev. A
Calibration
Problems
Sample arm does not go down
smoothly. The 96-well plate is incorrectly
seated in the XYP. Adjust the 96-well plate.
The 96-well plate is warped. Inspect the 96-well plate. Replace
it if it is warped.
The sample arm is misaligned. Readjust the sample arm
horizontal alignment.
The analyzer is misaligned with
the XYP instrument. Reposition the Luminex 100
analyzer on top of the Luminex
XYP instrument so that the holes
match up correctly. The
alignment guide should tighten
and loosen fairly easily if the
instruments are aligned correctly.
Continue to square the
instruments until the guide
loosens and tightens easily.
The sample probe is bent. Remove the sample probe from
the Luminex 100 analyzer. Roll it
on a flat surface. If it does not roll
smoothly, replace it with a new
sample probe.
Calibration is slow or fails There is a bad link between the
PC and the analyzer. Verify that all instruments
turned on and all
communications cables are in
place on back of instruments.
The calibration microspheres
are not fully suspended. Vortex the calibration vials to
resuspend the microspheres.
Wrong calibration lot chosen in
the Calibration Setup window. In the Main Window of the
Luminex Data Collector
software, click Calibrate. In the
Start Calibration dialog box,
make sure that the correct lot
numbers are shown in both the
Classification Channel and
Reporter Channel menus.