Luminex 100 User Manual Version 1.7 - Manual (Page 104)

Luminex 100 User Manual Version 1.7 x MAP Technology
8 - 10 PN 89-00002-00-063 Rev. A
Bead Detail
Irregularities
You can diagnose many problems by using the histogram described
on page page 6-17 or the Bead details box described on page page 6-
20.
A normal bead detail display is shown below. It depicts a tight bead
population within a white region.
Figure 8-1. Normal Bead Detail
The histogram for the bead detail above looks like this:
The Acquisition volume is set
too high. Set the acquisition volume to at
least 25 µL less than the actual
volume in your wells. This
setting lets the analyzer acquire
sample more efficiently with
less chance of acquiring air.
The xMAP microspheres are not
fully suspended. Vortex or pipette your samples
up and down to ensure that the
beads are present in the
solution.
You are using photobleached
microspheres. Replace photobleached
microspheres with a fresh lot of
microspheres