Luminex 100 User Manual Version 1.7 - Manual (Page 11)

PN 89-00002-00-063 Rev. A 1 - 1
Introduction
The Luminex ® 100 system is a compact analysis unit consisting of a
Luminex 100 analyzer, the Luminex XY Platform (Luminex
XYP™), a PC, Luminex software, and reagents. The Luminex 100
analyzer integrates optics, fluidics, and advanced signal processing in
a 17 inch by 20 inch footprint. The Luminex XYP provides efficient
handling of 96-well microtiter plates. The PC uses Luminex software
to control the analyzer. The optional Luminex Sheath Delivery
system (Luminex SD™) is used to provide sheath fluid directly from
a 20 liter container.
Photodiodes and a photomultiplier tube receive fluorescent signals
from xMAP
® microspheres. The analyzer digitizes the waveforms
and delivers the signals to a digital signal processor (DSP).
Proprietary algorithms function with the DSP to greatly increase the
sensitivity.
Hardware and preloaded software simplify installation and setup, and
with the help of this manual, you will be able to begin using xMAP
technology.
Principle of
Operation
The Luminex 100 system analyzes immunoassays, complex genetic
analyses, and enzymatic assays in one simple format. The reactants
(antibodies, oligonucleotides, substrates, etc.) of these assays are
anchored to the surfaces of uniquely fluorescent microscopic beads,
called microspheres. The unique fluorescent emission spectra of a
given microsphere identifies each of the assays performed
simultaneously on a single sample.
The Luminex 100 analyzer individually analyzes xMAP
microspheres in a flow stream. Every xMAP microsphere is
accurately classified to its own subset, based on its fluorescent
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