Luminex 100 User Manual Version 1.7 - Manual (Page 14)

Luminex 100 User Manual Version 1.7 x MAP Technology
1 - 4 PN 89-00002-00-063 Rev. A
Fluorescence
Compensation
Fluorescent spillover occurs when an emission spectrum overlaps
other emission criteria; therefore, selective filtering cannot occur. In
most analyzers, emission spillover is corrected using a technique
called compensation. Compensation involves subtraction of emission
percentage from another emission signal. In the Luminex 100
system, compensation is not necessary—the reporter signal does not
spill over into classification emissions.
Repetitive Microsphere
Measurements
Bound to reagents at the surfaces of the xMAP microspheres, the
reporter provides raw analytical data. Because a microsphere
suspension provides near liquid phase reaction kinetics, each
microsphere theoretically binds an equal number of reporter
molecules. Equal binding results in a statistically even distribution of
reporter on each microsphere in a set. When the Luminex 100
analyzer registers numerous events for each set, the system reports
individual population values based on an even coating of reporter.
This means that numerous replicates for each microsphere population
are measured from a single reaction vessel. The confidence in a
given measurement strengthens with increased replicate
measurements. For adequate confidence, 100 collected events per
microsphere set in each reaction vessel is usually sufficient.
Measurements are reported as the median for the xMAP
microspheres having a specific color signal.
About this Manual
This manual introduces you to the Luminex 100 system. With step-
by-step instructions, the manual guides you through initial setup and
powering-on procedures. Next, it offers a concepts section which
describes some of the key topics encountered during system
operation. Once you have a working knowledge of the analyzer, the
manual’s text and figures lead you through the data collection
process, giving detailed explanations of available options and
offering examples when necessary. Glossary and troubleshooting
sections assist as references. In short, this manual instructs you
through each operational stage—from unpacking the Luminex 100
analyzer and XYP to collecting your data.
The conventions in this document assume a basic familiarity with
computers and a knowledge of Windows
®
software. We typically
document only one method of accessing a command, although many
commands are available through more than one method, such as