Luminex 100 User Manual Version 1.7 - Manual (Page 63)

x MAP Technology Data Acquisition
PN 89-00002-00-063 Rev. A 6 - 13
Clear All: Clears the screen of all data and graphs. This function
deletes all associated files. A confirmation dialog displays when you
click this button to make sure that you intend to delete the table,
graphs, and the data files for the session.
Autosize: Adjusts column widths to fit the data and header sizes.
Statistic: This dropdown list offers a choice of statistic types
displayed in the table. Changing the statistic type does not affect
collected or recorded data.
Trimmed: Select this option to trim the top 5% and bottom 5% of
the collected data.
Prep: Initiates a pre-acquisition mode that allows you to prepare the
instrument for a session. In this mode, data is displayed but not
saved. You can use this function to optimize instrument settings
(such as gates and regions) before beginning a session. There is no
event number to indicate the end of a sample. Acquisition ends when
you click Cancel or when all of the sample is processed.
Start: Initiates a sample read. The sample probe lowers, draws
sample out of the well, and retracts to its original position. The
sample moves from the probe to the sample loop. If you click Start
before warming up the analyzer, an automatic 30-minute warmup
phase begins.
If you click Start without a sample present in the well, the analyzer
draws air into the sample loop and injects it into the flow chamber.
As a result, bubbles form in the cuvette and interfere with analysis. If
you click Start without a sample present, complete a 70%
isopropanol or 70% ethanol Alcohol flush cycle, followed by two
distilled water Wash cycles before acquiring further data.
Cancel: When the sample test begins, the Start button becomes a
Cancel button. The sample acquisition ends when you select Cancel
or if the entire sample is analyzed before the desired number of
events has been collected. At the end of sample acquisition the
sample probe lowers, then the syringe pump purges the sample loop
and probe with sheath fluid, sending a fraction of sheath fluid back
into the sample well.
Single: Check this box to run one sample at a time. When you
check the single check box, you need to start each run individually.
Note: To avoid the formation of air
bubbles in the cuvette, only click
Start when a sample is present.