Luminex 100 User Manual Version 1.7 User Manual

Page 103

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MAP Technology

Troubleshooting

PN 89-00002-00-063 Rev. A

8 - 9

The sheath or waste lines are
not fully connected.

Check to make sure all tubes are
tightly connected

The Calibration microspheres
have expired.

Replace old microspheres with a
fresh lot.

The calibration lot number
selected in setup is incorrect.

In the Main Window of the
Luminex Data Collector
software, click Calibrate. In the
Start Calibration dialog box,
make sure that the correct lot
numbers are shown in the
Calibration Channel menu.

Target values for chosen
calibration lot number are
incorrect.

Open the Start Calibration
dialog box. Highlight the lot
number, then click Delete. Re-
enter the calibration lot
information.

The wrong wells are selected in
the Setup XY tab.

On the Options Menu of the
Luminex Data Collector
software, click Options. When
the Options window opens,
click on the Setup XY tab. In
the Default Sample Locations
group box, make sure that the
correct corresponding wells are
chosen from the drop-down list.

Slow or Unsuccessful Sample
Acquisition

The air pressure is out of range.

See Pressurization on page 8-3.

The sample probe is clogged.

Clean the sample probe. See
page 7-4.

The sample probe is not
vertically aligned.

See Adjusting the Sample Probe
Height on page 4-4.

Air is present in the system.

Run three Prime commands,
then resume sample acquisition.

You are using an incorrect bead
set.

In Settings window, make sure
that the correct bead set is
selected. If the selected bead set
is not present in every well,
select “Total” events instead of
“Per Bead” events.

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