Luminex 100 User Manual Version 1.7 - Manual (Page 105)

x MAP Technology Troubleshooting
PN 89-00002-00-063 Rev. A 8 - 11
Figure 8-2. Normal Histogram
Symptom Possible Problem Solution
Calibration microspheres
classify too high You may be using
photobleached calibration
microspheres. Replace the microspheres with a
fresh batch. To avoid
photobleaching, protect your
microspheres from light.
xMAP microspheres hit
toward the lower right of the
region You may be using
photobleached xMAP
microspheres. Replace microspheres with a
fresh batch. To avoid
photobleaching, protect your
microspheres from light.
Scattered beads There is air in the system. Verify sample probe height, run
2-3 Alcohol Flush commands.
There is no sheath fluid in the
sheath bottle. Make sure there is sheath fluid
in the sheath bottle. Prime the
system until all air is out of the
system.