Luminex 100 User Manual Version 1.7 User Manual

Page 101

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MAP Technology

Troubleshooting

PN 89-00002-00-063 Rev. A

8 - 7

The wrong wells are selected in
the XY tab in the Luminex Data
Collector software.

On the Options Menu of the
Luminex Data Collector
software, click Options. When
the Options window opens,
click on the Setup XY tab. In
the Default Sample Locations
group box, make sure that the
correct corresponding wells are
chosen from the drop-down list.

The sample probe height is
incorrect.

Adjust the sample probe height.
See page 4-4.

You are using the wrong sheath
fluid.

Use only xMAP Sheath Fluid.

Calibration of Classification
Channel Does not complete

There is a bad link between the
PC and analyzer.

Verify that all instruments
turned on and all
communications cables are in
place on back of instruments.

The calibration microspheres
are not fully suspended.

Vortex the calibration vials to
resuspend the microspheres.

The wrong calibration lot was
chosen in the Calibration Setup
window.

In the Main Window of the
Luminex Data Collector
software, click Calibrate. In the
Start Calibration dialog box,
make sure that the correct lot
numbers are shown in both the
Classification Channel and
Reporter Channel menus.

The wrong wells are selected in
the XY tab in the Data
Collector software.

On the Options Menu of the
Luminex Data Collector
software, click Options. When
the Options window opens,
click on the Setup XY tab. In
the Default Sample Locations
group box, make sure that the
correct corresponding wells are
chosen from the drop-down list.

Classification channel
calibrates, but Reporter
channel fails.

Adjustments were made to
plates or sample probe height
after Classification calibration,
but before Reporter calibration.

Readjust plates or sample probe
to the original type or position.

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