LaMotte SMART3 Colorimeter - Manual (Page 81)

AMMONIA NITROGEN - LOW RANGE
SALICYLATE METHOD • CODE 3659-01-SC
QUANTITY CONTENTS CODE
60 mL *Salicylate Ammonia #1 *3978-H
10 g *Salicylate #2 *7457-D
2 x 5 g *Salicylate #3 Reagent Powder *7458-C
1 Spoon, 0.1 g, plastic 0699
1 Spoon, 0.15 g, plastic 0727
1 Pipet, 1.0 mL, plastic 0354
*WARNING: Reagents marked with an * are considered to be potential health
hazards. To view or print a Material Safety Data Sheet (MSDS) for these reagents
go to www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail,
phone or fax.
Ammonia nitrogen is present in various concentrations in many surface and ground
water supplies. Any sudden change in the concentration of ammonia nitrogen
in a water supply is cause for suspicion. A product of microbiological activity,
ammonia nitrogen is sometimes accepted as chemical evidence of pollution when
encountered in natural waters.
Ammonia is rapidly oxidized in natural water systems by special bacterial groups
that produce nitrite and nitrate. This oxidation requires that dissolved oxygen be
available in the water. Ammonia is an additional source of nitrogen as a nutrient
which may contribute to the expanded growth of undesirable algae and other forms
of plant growth that overload the natural system and cause pollution.
APPLICATION: Low concentrations of ammonia in fresh, brackish and salt
water; fresh and salt water aquariums.
RANGE: 0.00 - 1.00 ppm Ammonia-Nitrogen
MDL: 0.05 ppm Fresh Water
0.10 ppm Salt Water
METHOD: Salicylate and ammonia react at high pH in the presence
of a chlorine donor and an iron catalyst to form a blue
indophenol dye, the concentration of which is proportional
to the ammonia concentration in the sample.
SAMPLE HANDLE &
PRESERVATION:
Ammonia solutions tend to be unstable and should be
analyzed immediately. Samples may be stored for 24 hours
at 4°C or 28 days at –20°C.
INTERFERENCES: There are few interferences in most natural waters. High
concentrations of reducing agents, such as hydrazine,
react with the chlorine donor and can result in negative
interferences. Color and turbidity can also interfere.
SMART3 Test Procedures 11.10 AMMONIA NITROGEN, Low Range
Test P
rocedures