Metrohm 761 SD Compact IC - Manual (Page 47)

2.15 Precolumn and separating column
761 SD Compact IC / Instructions for Use 8. 761.1043
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2.15 Precolumn and separating column
2.15.1 Metrosep RP Guard
Using the precolumn (Metrosep RP Guard 34) with easily exchangeable
filters serves to protect the separating column 36 and considerably pro-
longs its service life.
The precolumn has two connections for PEEK capillaries and must be
fitted as follows:
1 Remove the PEEK coupling between column con-
nection capillary 33 and suppressor
• Undo the PEEK coupling 54 fitted in Section 2.6.4 between
column connection capillary 33 and suppressor and remove
it. It was fitted only for rinsing without column (Section 2.14).
2 Connect the Metrosep RP Guard 34
• Detach the screw caps from the Metrosep RP Guard.
• Attach a PEEK pressure screw 50 to the column connection
capillary 33.
• Firmly screw the precolumn to the column connection capil-
lary 33 (the arrow on the precolumn must point in the flow di-
rection).
• Connect the connection capillary 35 belonging to the precol-
umn to the other end of the precolumn.
3 Prepare for rinsing the precolumn
• Route the free end of connection capillary 35 connected to
the precolumn 34 into a beaker.
• Open the startup.smt system and connect it as described in
Section 2.13.1.
• Double-click on the 761 image in
the system window. You will see
the window for manual operation
of the 761 SD Compact IC (see
below):
4 Rinse the precolumn
• Switch on the high-pressure pump ( IC pump ) by clicking on
<On>, rinse the precolumn for approx. 10 minutes with eluent.
• Switch the high-pressure pump back off again by clicking on
<Off> .
Next connect the Metrosep A Supp 1 HS separating column 36, see
Section 2.15.2.