Metrohm 761 SD Compact IC - Manual (Page 48)

2 Installation
761 SD Compact IC / Instructions for Use 8. 761.1043
40
2.15.2 Metrosep A Supp 1 HS separating column
Now fit the supplied Metrosep A Supp 1 HS as the actual IC separating
column.
When fitting the column, always ensure that it is fitted correctly as
shown by the flow direction on the sticker (the arrow must point in the
flow direction). Ensure that the set flow rate is not higher than the per-
mitted flow rate for the corresponding column (see the sheet en-
closed with the column).
The separating column 36 has two connections for PEEK capillaries
and must be fitted as follows:
1 Connect the separating column 36 downstream of
the precolumn 34
• Detach the screw caps from the Metrosep A Supp 1 HS
separating column 36.
• Screw the inlet end of the separating column 36 (note the
flow direction, the arrow on the sticker must point in the flow
direction) to the free end of the connector 35 attached to the
precolumn 34.
2 Prepare for rinsing the separating column
• Position the beaker beneath the column outlet.
• Open the startup.smt system and connect it as described in
Section 2.13.1.
• Double-click on the 761 image in
the system window. You will see
the window for manual operation
of the 761 SD Compact IC:
3 Rinse the separating column
• Switch on the high-pressure pump ( IC pump ) by clicking on
<On> and rinse the column for approx. 10 min with eluent.
• Switch the high-pressure pump back off again by clicking on
<Off> .
4 Connect the separating column to the suppressor
The column outlet is connected to the eluent inlet of the sup-
pressor so as to close the eluent path:
• Fit a PEEK pressure screw 50 to the "suppressor inlet capil-
lary for eluent ("Eluent")" 37.
• Then screw this pressure screw into the column connection
of the separating column 36.
5 Fix the separating column in position
• Insert a column holder (6.2027.040) 67 into the mounting rails
66 and secure the separating column 36 in the column hol-
der.