Bio-Rad BioFrac™ Fraction Collector - Manual (Page 23)

Fraction Collector Setup
User Guide | 15
To set up the BioFrac fraction collector
1. Place the fraction collector on a level surface on a laboratory or coldroom
bench or in a cold cabinet. Alternatively, if benchspace is limited, the modular
fraction collector can be stacked on top of the NGC, BioLogic DuoFlow™, or
BioLogic™ LP chromatography system workstations.
2. Connect the fraction collector display cable to the port labeled Display on the
back of the fraction collector. (See
Figure 5 on page 10 .)
3. Connect the diverter valve cable to the Diverter Valve port on the back of the
fraction collector. (See
Figure 5 on page 10 .)
4. Attach the diverter valve to either the right or left column. The diverter valve
should be attached so that the Collect port is pointed up and Waste port is
pointing down (see
Figure 6 on page 14 ). Use the included hex key to secure
the diverter valve to the column.
5. Connect all power cords to available grounded, surge-protected outlets.
6. Connect the preassembled 26-inch PEEK 1/16" OD tubing, supplied in the
BioFrac fittings kit, to the Collect port of the diverter valve and to the drophead.
Use the 0.02" ID (orange) tubing for flow rates less than 20 ml/min and the 0.03"
ID (green) tubing for flow rates greater than 20 ml/min.
Note: The diverter valve is rated to a maximum pressure of 30 psi.
If tubing other than that found in the fittings kit is used to plumb the fraction
collector, make sure all tubing lengths are kept to a minimum. This reduces the
delay volume (see Collect Using a Delay Function on page 67 ) and
backpressure. Tubing choice is dependent on the flow rate and pressure
characteristics of the pumping system. For flow rates above 20 ml/min, use
0.03" ID or 1/8" OD, 0.062" ID tubing (available from most tubing/fitting
suppliers).
7. Attach the 0.03" ID Tefzel tubing, supplied in the fraction collector fittings kit, to
the Waste port of the diverter valve and place the other end into a waste
collection vessel.
Note: If using flow rates greater than 20 ml/min, be sure to use tubing that
has a 0.03" or larger ID.