Bio-Rad BioFrac™ Fraction Collector User Manual
Page 74
5
| Stand-Alone Operation
66
| BioFrac Fraction Collector
number and end tube number are automatically updated according to the
MultiRun mode selected.
4.
Set the fraction size units (min, ml, drops) and set the fraction size. If collecting
by volume, you must enter a flow rate.
5.
Press Collect (F4) and turn Threshold on. Set the global %AUFS (absorbance
units full scale) and set the detector input voltage to either 100 mV or 1 V full
scale depending on your detector’s output voltage. Press Done.
On the Main screen, Collect should now read Collect –>Threshold.
6.
For Collection of non-peak fractions, change NonPeak Frac. from Divert to
Collect and enter a size for the non-peak fractions.
7.
(Optional) Set the bubble filter time. This function detects and filters false peaks
(such as electrical noise or air bubbles). Typically, a bubble filter time of 0 or 1
sec will suffice. (For discussion of the bubble filter time function, see
8.
(Optional) Press Collect (F4) and set the Delay time (see
).
9.
(Optional) Press Method (F3) and save your method.
10. Press Run (F1) to start the experiment.
At any point during the run you can use the Run screen function keys (see
).