Bio-Rad BioFrac™ Fraction Collector - Manual (Page 38)

3 | System Configuration and Plumbing
30 | BioFrac Fraction Collector
Ice Bath
Remove the lid of the ice bath/microplate rack and fill the tub approximately ½ full
with crushed ice, replace the lid and insert 13 x 100 mm culture tubes. Remove the
rack positioning tray from the fraction collector and replace it with the ice bath rack.
Adjust the fraction collector control module appropriately for the height of the tubes
being used. The height adjustment lock pins (see Figure 11 on page 32 ) can be used
to lock the drophead at the desired height. Tube number 1 is located in the front, left
corner of the rack.
Microplates and Titertube Tubes (Racks P1, P2, P3, P4, and TT1)
Remove the rack positioning tray from the fraction collector and replace it with the
ice bath/microplate rack. Position the ice bath/microplate rack such that rack tube
number 1 is in the left, front corner. Mount the plates on the rack using the plate
positioning tabs located on the top of the rack. Adjust the fraction collector control
module appropriately for the height of the plates being used. The height adjustment
lock pins (see Figure 11 on page 32 ) can be used to lock the drophead at the
desired height. Position the plates such that tube A1 is in the left, front corner. In
order to obtain a uniform fraction size when collecting fractions smaller than 0.5 ml,
we strongly suggest that fraction size be specified in drops rather than time or
volume. Alternatively, the microplate Drop Head Kit (catalog # 741-0088) can be
used to obtain finer resolution of fraction sizes since it delivers an approximately
25
l drop.
Preparative (Prep-20) Adaptor
Attach Tygon tubing to each funnel that is long enough to reach the collection
vessels (20 feet of 3/8" OD, 1/4" ID Tygon tubing is provided for this purpose.)
Ensure that no kinks constrict flow in the tubing. Gravity flow from the prep adaptor
requires that the container used for collection be mounted below the Prep-20
adaptor funnels. A drain trough is provided as added security in the event the tubing
becomes plugged or kinked, preventing sample loss. The drain trough tubing should
be inserted into a clean empty collection vessel.