Example: collection using time windows and delay – Bio-Rad BioFrac™ Fraction Collector User Manual

Page 81

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Collect Using a Delay Function

User Guide

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Example: Collection Using Time Windows and Delay

Case 1, where start time = 5 min and delay time = 0 min
Case 2, where start time = 5 min and delay time = 0.25 min

Assume the following:

Note that each time or volume window is separated by a tube containing the delay

volume. If delay time is zero, then tube #1 will be empty and subsequent time or

volume windows collected are separated by empty tubes.

Fig. 30.

Collection windows and a delay.

Flow rate

1 ml/min

Peak fraction size

1 min

Time window #1

Start 5, End 8

Time window #2

Start 10, End 12

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