Bio-Rad BioFrac™ Fraction Collector User Manual
Page 70
5
| Stand-Alone Operation
62
| BioFrac Fraction Collector
The fraction collector stops when the last tube is reached unless a stop
command is received before it reaches the last tube. At the end of a run, the
start tube number and end tube number are automatically updated according
to the MultiRun mode selected.
4.
Set the fraction size units (min, ml, drops) and set the fraction size. If collecting
by volume, you must enter a flow rate.
5.
Press Collect (F4) and turn Threshold on. Set the global %AUFS and set the
detector input voltage to either 100 mV or 1 V full scale depending on your
detector’s output voltage. Press Done. On the Main screen, Collect should now
read Collect –> Threshold.
6.
For collection of non-peak fractions, change NonPeak Frac. from Divert to
Collect and enter a size for the non-peak fractions.
7.
(Optional) Set the bubble filter time. This function detects and filters false peaks
(such as electrical noise or air bubbles). Typically, a bubble filter time of 0 or 1
sec will suffice. (For a discussion of the bubble filter time function, see
Appendix A, Bubble Filter Time
8.
(Optional) Press Collect (F4) and set the delay time.
9.
(Optional) press Method (F3) and save your method.
10. Press Run (F1) to start the experiment.
At any point during the run you may use the Run screen function keys (see
).