Bio-Rad BioFrac™ Fraction Collector - Manual (Page 84)

6 | LP/Econo Mode Operation
76 | BioFrac Fraction Collector
advances. If you are collecting in microplates or Titertube tubes choose a
collection pattern: Serpentine, Row, or Column.
Note: Whenever a new rack is selected, the start tube and end tube values
are updated to reflect the maximum number of tubes available for the
selected rack.
4. Choose the appropriate MultiRun mode for your experiment (see Table 6 on
page 39 for a description of the MultiRun function).
5. Set the start tube and end tube rack position and tube number as follows:
 If you are overlaying fractions, set the start tube and end tube numbers on
the fraction collector to correspond to the required number of fractions
derived from programming the fraction size on the BioLogic LP system, the
Model EP-1 Econo pump, or other controller. For example, assuming the
number of fractions calculated is 30:
 If start tube number is A1, then end tube number is A30
 If start tube number is B16, then end tube number is B45
 If you are doing sequential fraction collection, set Multi Run = Seq. Tube +1
or Seq. Rack and set the start tube and end tube numbers on the fraction
collector to be greater than or equal to the number of fractions required for
the run. When the run is ended by pressing Stop or by reaching the last
tube, the BioFrac fraction collector will automatically increment start tube
and end tube according to the MultiRun function selected (see Table 6 on
page 39 ).
 Alternatively, sequential tube collection can be controlled by the
BioLogic LP chromatography system. In this case, however, no tubes are
skipped between runs. To run in this mode, set MultiRun to Seq. Tube+1 or
Seq. Rack and set end tube to be greater than or equal to the total number
of tubes required for all runs. For example, if you will be doing three runs
requiring 25 tubes each, then 75 tubes are required. Set the MultiRun
parameter on the BioLogic LP system as described in the BioLogic LP
system manual.