Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 122)

Luminex 200 User Manual for LDS Version 1.7
x
MAP Technology
9 - 4 PN 89-00002-00-150 Rev. A
xMAP Microsphere
Stability and Storage
xMAP microspheres are light sensitive and should be protected from
light during all stages of storage and usage. In addition, freezing
conditions and organic solvents should be avoided.
Two-Step
Carbodiimide
Coupling of Protein
to xMAP
Carboxylated
Microspheres
Introduction
Use the protocols included here as a general starting point for
developing assays. All assays should be optimized for your reagents
in your specific application. You will discover the methods that give
you the best results by starting with these guidelines and modifying
them for your specific needs.
Equipment
• Vortex
• Sonicator bath
• Micropipetters (1 µl - 1000 µl)
• Microcentrifuge
• Analytical balance
• Timer
• Rotator
Materials
• xMAP carboxylated microspheres—LIMIT EXPOSURE TO
LIGHT!
• Microcentrifuge tubes: 1.5 ml, polypropylene (See Technical
note 4)
• ACTIVATION BUFFER:
0.1 M Monobasic Sodium Phosphate (pH 6.2 ± 0.2)
• COUPLING BUFFER:
50 mM MES (pH 5.0 - 6.0)
• WASH BUFFER:
Phosphate Buffered Saline (pH 7.4 ± 0.1), Tween
®
20
(0.05% v/v)
• BLOCKING/STORAGE BUFFER:
Phosphate Buffered Saline (pH 7.4 ± 0.1), Bovine Serum
Albumin (10 mg/mL), Sodium Azide (0.05% w/v)