Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 90)

Luminex 200 User Manual for LDS Version 1.7
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MAP Technology
7 - 2 PN 89-00002-00-150 Rev. A
Startup
‹
Perform the entire startup procedure on a daily basis.
1. Turn on the analyzer and PC.
2. Click Warmup.
3. While you are waiting for the analyzer and optics system to
warm up - about 30 minutes - check the levels of sheath fluid and
waste fluid. Check that the sheath container cap is tight.
4. Prime the system.
5. Perform an Alcohol Flush with 70% isopropanol or 70%
ethanol.
6. Wash twice with distilled water or sheath fluid. For optimal
performance, Calibrate the system and then Wash four times
with distilled water. See page 5-2 for more information about
calibration.
Shutdown
‹
Perform the shutdown procedure when you finish running
samples.
1. Dispense 1.2 mL of a fresh 10%-20% bleach solution into the
Luminex XYP reservoir. Run a Sanitize command.
2. Run the Wash command twice with distilled water.
3. Run a Soak command with distilled water.
4. Raise the sample probe.
5. Click Eject on the toolbar to eject the plate holder.
6. Remove the microtiter plate and the reservoir.
7. Clean the plate holder and reservoir with a 10%-20% bleach
solution.
8. Replace the reservoir, then click Retract on the toolbar to retract
the plate holder.
9. Loosen the sheath fluid container cap to release pressure in the
system.
10. Turn the analyzer and PC off.
Note: There is NO sensor to warn
you of low sheath fluid levels. Fill
the sheath fluid container before
the level reaches the sheath outlet
line. There is NO indicator light to
warn you of high waste volume.
Empty the waste container each
time you fill the sheath container.