Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 53)

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MAP Technology System Startup
PN 89-00002-00-150 Rev. A 5 - 7
The progress bar shows the calibration progress, which should
take less than two minutes. If the status changes to “Sample
Empty” before calibration completes, a “Calibration Failed”
message appears. Click OK and restart the process. When
calibration finishes for the classification microspheres, a
“Calibration Succeeded” message appears. The settings and any
comment you enter are logged into the calibration report.
15. Click OK in the confirmation dialog box. The Calibration dialog
box opens again.
16. Select Reporter and click Start. The progress bar shows you the
calibration progress. Calibration should take less than two
minutes. If the status in the system monitor changes to “Sample
Empty” before calibration completes, a “Calibration Failed”
message appears. Click OK and start the process again. When
calibration finishes for the reporter microspheres, a “Calibration
Succeeded” message appears. The settings and any comment you
enter are logged into the calibration report.
17. Click OK on the confirmation dialog box.
xMAP Calibration microspheres are very concentrated. Each
time you calibrate the instrument, follow with 4 wash cycles
using distilled water.
18. Click Eject from the toolbar and remove the plate.
19. Click Retract to retract the plate holder.
Preview or Print
Calibration Report
The calibration report is a quality control tool that shows the
frequency of past calibrations, operators of past calibrations, and
trends in instrument settings. The calibration report is available as a
log or as a detailed report.
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To print or preview the calibration report:
1. Close all open sessions.
2. Click Print on the toolbar. The Print dialog box opens.