Luminex 200 User Manual with LDS 1.7 Software - Manual (Page 56)

Luminex 200 User Manual for LDS Version 1.7
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MAP Technology
5 - 10 PN 89-00002-00-150 Rev. A
if a Luminex XYP instrument is added to the system. See page 4-3
for more information.
Sanitize: Decontaminates the sample lines and cuvette after
biohazard contact. Use a 10%-20% bleach solution to sanitize the
system after running biohazardous samples. See page 7-3 for the
sanitizing procedure.
Prime: Removes air pockets from the fluidic pathways. Use this
operation after system installation and following replacement of the
sheath fluid. Sheath fluid flows freely for several seconds followed
by a fill-and-purge action by the syringe. Repeat until the waste line
outside the instrument appears free of all significant air pockets.
Alcohol flush: Removes air bubbles from the walls of the cuvette.
Use 70% isopropanol or 70% ethanol for the alcohol flush solution.
After an alcohol flush, run 2 wash cycles with distilled water.
Wash cycle: Removes residual sample from the sample acquisition
lines. Place at least 200 µL of distilled water in the Luminex XYP
reservoir. After calibration, run 4 wash cycles with distilled water.
Back flush: Removes obstructions from the cuvette. Use this if
fluid does not flow through the waste tubing during prime cycles or
during sample acquisition. Routine back flushes are not
recommended; use back flush only as a troubleshooting method.
Drain: Temporarily drains the cuvette, then does a fill command.
Useful for cleaning debris buildup in the cuvette, or for clearing
bubbles from the cuvette. Routine drains are not recommended.
Sheath fluid is expelled at the end of the drain function.
Soak: Holds a solution in the fluidics lines for an extended time.
When shutting down the instrument overnight or for an extended
period of time, use a distilled water soak. Salt in the sheath fluid can
form salt crystals in the sample probe or instrument tubing. When
restarting the instrument, follow the routine startup procedure shown
on page 7-2 to re-equilibrate the system with sheath fluid. Before you
use the Soak command, place 1.2 mL of soak solution in the
Luminex XYP reservoir.
Warmup: Performs an initial 30-minute optics warmup. While
powered, the optics remain engaged for 4 hours after each
acquisition. After 4 hours of inactivity, the lasers automatically shut
off, and must be warmed up again before the next acquisition. If Start
is activated before warming up, an automatic 30-minute warmup
phase is started. The warmup phase can be cancelled using the
Cancel warmup command on the Analyzer menu. We strongly
Note: Always follow a Back flush
with a 70% isopropanol OR a 70%
ethanol Alcohol flush. Follow the
Alcohol Flush with two distilled water
or sheath fluid wash cycles.