3 roboinject, 1 welcome to the roboinject – Multichannel Systems Roboinject Manual User Manual

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3 Roboinject

3.1 Welcome to the Roboinject

The Roboinject is a fully-automated all-in-one solution for injection of cDNA, mRNA and other
compounds into Xenopus oocytes in standard 96 well plates. It is also suited for automated
injection of other cells or organisms in standard (96, 384) as well as customized well plates.

Main features:

 Compact and functional design, works fully computer controlled.

 Easy handling - neither special skills nor special equipment required.

 Maintenance-free system.

 Extremely simple and stable mounting of injection capillaries.

 Injection is performed by a positive displacement motorized unit which is capable

of repetitively dispensing precisely 1 to 100 nl per cell or organism.

 Reproducible and defined cell impalement depth.

 Automated sample uptake of up to eight different RNAs, DNAs or compounds

per well plate from industry standard 0.5 ml reaction tubes.

 Automated and effective needle rinsing before uptake of new samples.

 Injection of up to four different samples per cell.

 Full documentation of all injection parameters.

 Compatibility with the Roboocyte when using 96 well plates.

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