Multichannel Systems Roboinject Manual - Manual (Page 26)

Roboinject Manual
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Click "Inject" tab.
The dialog allows to define parameters concerning the “Injector Mount” (z-axis) and the
“Injector” device itself. The settings are attributed to the different "Sample Types" such
as RNA, DNA or others.
“Sample Type”: Define the type of compound to which you want to assign the setting
parameters from the drop down menu.
“Injector Mount = z- Axis”:
You can separate the impalement process of the injection needle into an oocyte or another cell
in two phases. This is especially useful, for example, when working with "soft" oocytes. If the
first movement (for example, to 650 μm above well bottom) only pushes down the cell without
penetrating the vitelline layer and membrane, a second movement (for example, to 550 μm
above well bottom) will most likely lead to a successful impalement. Please see the picture.
Please define the parameters of the first step, called “Impalement Position”. Please note that
the value in micrometer refers to the distance between the tip of the needle and the well bottom.
Define the “Velocity” of impalement in mm/s. The default value of 30 mm/s was intensively
tested with oocytes and will work in most of the cases.
Please define the parameters of the second step, called “Injection Position”. Define the position
of the injection in μm from the up-down control. Please note that the value in micrometer refers
to the distance between the tip of the needle and the well bottom in relation to the bottom of
the well. Define the “Velocity” of injection in mm/s. Again, the default of 30 mm/s will work with
most oocytes.