Multichannel Systems Roboinject Manual - Manual (Page 37)

Roboinject
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Injector = Plunger Section
Click "Reference". The plunger will perform its reference movement and will stop in home
position.
Click "Home" to move the injection plunger inside the z-axis into home position. The home
position is defined as the topmost position.
Click "Replace" to start the needle installation or needle changing.
Click "Empty" to move the injector plunger continuously from the topmost position into the
injection needle to extrude the oil filling of a new injection needle.
Click "Aspirate" to move the injector plunger upwards for aspirating the volume in nl selected
from the up-down control beside the button. Be sure to immerse the tip of the needle into the
medium you want to aspirate to avoid the suction of air into the needle.
Note: You cannot aspirate more "volume" than it has been ejected before by the command
"Empty".
Click "Inject" to move the plunger stepwise from top to bottom, for injecting the volumes
selected from the up-down control beside. It is possible to inject volumes from 1 to 100 nl.
Changing the Injection Needle
Click on the icon to open the "Manual Mode" dialog.
Use the injector section to test the functionality of the injector with installed injection needle to
test the injector under more realistic conditions or to do injections manually. Click "Home" and
wait until the plunger arrived at its topmost home position. Click "Replace" in “Injector Section”
of “Manual Mode” if there is an used needle installed, and remove it carefully. Be sure not to
bend the plunger wire.
Fill a fresh injection needle completely and air-bubble free with mineral oil. Install the needle by
inserting the plunger wire end slowly into the capillary. Lock the luer connector tightly but not
with excessive force.