Multichannel Systems Roboinject Manual User Manual
Page 55
Injection
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Preparing the sample for injection
1. Take the sample out of the freezer at least 10 minutes before the injection.
2. Let it stand at room temperature until the sample is completely thawed.
3. Mix the sample thoroughly, for example, by vortexing for a short moment,
or manually by striking the side of the tube with a finger.
4. Spin the sample in an Eppendorf centrifuge (or a similar centrifuge) at full speed (>10000 g)
for 5 minutes immediately before the injection for sedimentation of any remaining particles
that might clog the needle.
Filling and placing of the reaction tubes
3.
Please use the provided reaction tubes as sample reservoirs.
4.
Fill the respective reservoir tubes (position 1 to 8) always with a sample volume that is 1 μl
more than needed for the injection of all selected oocytes. The needle tip will not go down
to the very well bottom but will always stop at a safety distance. This avoids breakage and
contamination of the tip.
5.
Fill the two rinsing reservoirs (position 9 and 10) with at least 200 μl of nucleotidase-free
water.
6.
The waste reservoir (position 11) can be kept empty, it will be used for oil removal only.
7.
Place the tubes centered into the mounting holes. Bend the tube caps in a way that they
are perpendicular to the tube. The tube caps should be positioned that they are facing
away from the plate.