Multichannel Systems Roboinject Manual - Manual (Page 63)

Injection
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Changing or loading the injection needle
1. Use the "Change Needle" command to change or install a new needle. "Change Needle" will
move the plunger and carrier in home position and move the z-axis a bit down for better access
to the luer connector for the needle.
2. Use a fine pipette tip, for example, a microloader filled with mineral oil. Insert the fine tip into
the needle as close as possible to the needle tip. Fill the needle completely and air-bubble free
with oil. The remaining air in the tip region will be cleared automatically later on.
3. Insert the plunger wire carefully into the needle end, move the needle slowly up and close the
luer lock. Part of the oil will drop out of the needle tip. Remove it with a soft tissue from the
table. Be careful not to break the glass capillary.
Reset needle to coarse position
Enable the check box "Reset Needle Coarse Position" if the needle length has changed since
the last alignment.
During the alignment process, the injector mount (z-axis) is moved automatically to the last
alignment position minus a safety distance of 3 mm (so called "Coarse Position"). When using
a needle that is longer than the one used last time, there is a risk that the needle will break.
You should reset the memorized "Coarse Position" in this case. The z-axis will not move down
during the automatic coarse positioning and the needle will be safe.
Click the "Next" button for displaying the "Alignment" dialog of the "Start Injection Wizard".