Multichannel Systems Roboinject Manual - Manual (Page 62)

Roboinject Manual
58
4.6 Changing the Well Plate and Loading the Needle
You will need the cDNA or mRNA samples, the well plate loaded with oocytes, and an injection
needle. You can reuse injection needles several times as long as they are not clogged or damaged.
You will also need the provided stereo microscope and the provided alignment device (with
crosshairs) in order to position the well plate horizontally and vertically as well as the injection
needle before you start the injection. The alignment wizard will lead you through all necessary
steps.
Note: Make sure that all oocytes have had time to settle for several hours and are positioned in
the well with the animal pole (dark side) up. See also "
Preparation of Xenopus Oocytes " in the
Appendix.
Warning: Do not move the z-axis by hand while installing the injection needle. In all cases,
perform an alignment after you have replaced the needle or the well plate.
1. Click the "Start" button in the main window. The "Start Injection Wizard"
"Preparation" appears.
2. Click "Change Plate" in "Plate Carrier" window. After loading or replacing the well plate that,
please click "Home". The carrier moves to home position (X = 0, Y = 0).
3. If necessary, remove the old well plate from the carrier.
4. Take a well plate from the type you specified in the "Plate Info" dialog. Place it onto the carrier
and press its side against the spring in the lower right corner of the carrier. Then put the rest of
the plate down onto the carrier. It should fit tightly and immovably now.
5. Put the alignment device (with crosshairs) in well H12.
6. Enable or deselect the check box "Reset Needle to Coarse Position".
7. Click "Change Needle" in "Injector Mount" window.