Multichannel Systems Roboinject Manual - Manual (Page 65)

Injection
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Open the “Alignment” dialog in the “Start Injection Wizard”.
1. Switch in "Plate Carrier" to "Fast". The movement of the z-axis and the carrier commanded
by the software controls will now be fast.
2. Move the injector mount (z-axis) and the carrier by pressing the arrow keys on the alignment
dialog until the needle is almost centered on the alignment device.
3. Switch to "Slow". The movement will now be carried out in small single steps.
4. Fine-tune the position until the alignment device is almost touched by the tip of the needle.
If you do this the first time, you may have difficulties seeing the needle three-dimensionally.
The needle may appear to be centered correctly, but instead the needle is still a way above the
alignment device and shifted to the front. If you do not feel sure about this, lower the needle
slowly and carefully.
Important: Do not touch the alignment device with the injection needle! The fine tip should
blunt and will not impale the oocyte or the needle will break.
Then, move it up again one step to optimize the position. However, you will see that after
a bit of experience, the alignment is performed very quickly and without trouble. If you need
to start the alignment again, for example, if you have moved the carrier accidentally by hand,
click "Back" to go back to the "Preparation" dialog, and "Next" to restart the alignment
process from step 1.