Multichannel Systems Roboinject Manual - Manual (Page 67)

Injection
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4.8 Starting the Injection
To start the injection after successful alignment, simply click the "Finish" button in the Alignment
dialog window. After the oil has been pushed out to the waste tube and the volume of the first
sample has been aspirated, the "Test Injection" dialog appears if activated.
This dialog gives the user the possibility to check the transmissibility of the tip opening of
the injection needle. The respective sample volume will be injected into air. Before doing so,
please place the microscope on the x/y table in a way that you can clearly see the needle tip.
Multi Channel Systems MCS GmbH recommends up to two test injections for checking the
transmissibility of the tip opening. The volume of compound needed for this feature is aspirated
in additional to the injection volume, defined in “Injection” tab of “Options” dialog in menu
“Settings”. You define, for example, 50 nl injection volume. Then 50 nl are additionally aspirated,
sufficient for two test injections with 25 nl each. If you do more than two test injections,
the missing volume is automatically taken up during the injection process.
Click the button "Inject" to inject the test volume of compound in nl which is displayed
in the up-down control window.
The "Sample" window displays important sample parameters. In the "Auto close" window
the time in seconds is counted down before this dialog is automatically closed.
Click "Continue" to start the injection process.
Click "Halt Time" if you need more time, for example, to check the tip opening by doing test
injections.