Multichannel Systems Roboinject Manual - Manual (Page 52)

Roboinject Manual
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4.2 Strategies for a Successful Injection
Multi Channel Systems provides injection pipettes with an inner diameter exactly matching the
plunger wire. The provided injection pipettes are glued to luer-fittings and therefore ready to use.
If you nevertheless decide to fabricate your own injection pipettes you should keep in mind that
you can only use the capillaries listed in the chapter "Ordering Information" in the Appendix.
Other capillaries will not work properly.
The Roboinject software allows to define the impalement and injection position for each sample
separately during the well selection process. The "Impalement Position" and the "Injection
Position" in the main "Set Injection" window denotes the distance of the needle tip to the well
bottom (zero value). This means, for example, that for an oocyte with a diameter of 1.2 mm an
impalement or injection position of 650 μm would correspond to an oocyte impalement depth
of 550 μm (1200 μm - 650 μm = 550) Please read chapter "Inject" tab of "Option" dialog in
"Settings" menu.
Changing and loading the injection needle
Click to start the “Manual Mode” dialog. Use the "Change Needle" command to change
or install a new needle. "Change Needle" will move the plunger and carrier in home position
and move the z-axis a bit down for better access to the luer connector for the needle.
Use a fine pipette tip, for example, a microloader tip filled with mineral oil. Insert the fine tip into
the injection needle as close as possible to the needle tip. Fill the injection needle completely and
air-bubble free with oil. The remaining air in the tip region will be cleared automatically later on.
Insert the plunger wire carefully into the needle end, move it slowly up and close the luer lock.
Part of the oil will drop out of the needle tip. Remove it with a soft tissue from the table.
Be careful not to break the glass capillary.