Multichannel Systems Roboinject Manual - Manual (Page 64)

Roboinject Manual
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4.7 Alignment
You have to adjust the position of the carrier and the needle under microscopic control.
The Roboinject always moves relative to a standard = home position. The aim of the alignment
process is to define this standard position. If this is not done properly, the Roboinject moves to
wrong positions. The alignment process also compensates for minor changes in the dimensions
of different well plates and variations in the length of the needle. To achieve a high expression
rate, it is essential to perform an alignment each time before you start an injection sequence.
Note: The alignment is very important for obtaining best results. An alignment has to be
performed each time before you start an injection. It is very important that the glass capillary
is accurately centered on the alignment device. Otherwise, the oocytes will not be properly
impaled and injected.
Warning: Hitting the alignment device can cause the injection needle to shatter, possibly
projecting splinters, which can be dangerous. Eye protection should be used, and this
alignment should never be observed up close with an unprotected eye.
Place the microscope in a position onto the x/y table which allows to focus on the alignment
device plugged in well H12.