Multichannel Systems Roboinject Manual - Manual (Page 40)

Roboinject Manual
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"Injection" Window
After completing the definition of the injection protocol the "Start Injection Wizard" proceeds
with the final preparations for the injection.
Via "Start" and "Break" buttons you start and stop the injection manually. The status bar under
the buttons will show the processing of the injection.
Click the "Start" button. The "Preparation" dialog of the "Start Injection Wizard" appears.
Air pressure: Please check the air pressure, the minimum has to be 3 bar = 3000 hPa.
Check the connection status: A green LED indicates a sufficient air pressure.
Plate Carrier: Click "Change Plate". This command will move the carrier to an easily accessible
position. Please remove the old well plate from the carrier if necessary, Take a well plate from
the type you specified in the "Plate Info" dialog box. Place the plate onto the carrier and press
it against the springs in the lower middle and lower right corner of the carrier. Then put the rest
of the plate down onto the carrier. The well plate position is correct when there is no play
between the well plate bottom and the carrier surface at all edges and when the well plate
has full contact to the left and upper side of the carrier holding frame.
Place the reservoir tubes according to their appearance in the virtual well plate in the respective
mounting holes. Do not use excessive force. Also place the rinse tubes and the waste tube at their
designated positions.
Click "Home" for moving the well plate carrier into the home position.
Injector Mount: Please mount an injection needle. Click "Change Needle". Enable the check box
"Reset Needle Coarse Position" to initialize the manual z-axis alignment after installation of
the injection needle, if the needle length has changed.
Put the alignment device (with crosshairs) into well H12. Please read chapter “Alignment”.