Multichannel Systems Roboinject Manual - Manual (Page 15)

Roboinject
11
Injection and cultivation of Xenopus oocytes is performed using disposable standard 96 well
plates, which are commercially available from several providers. Please see chapter "Ordering
Information" in the Appendix. The oocytes are plated into the wells in a couple of minutes and
can be kept for several days. They quickly settle within the cone-shaped wells and adhere to the
well bottom after a few hours. The oocytes do not have to leave the plate anymore; you can easily
transfer the oocytes from the incubator to the Roboinject and back again.
A quick alignment process guarantees that the oocytes are injected precisely. The cDNA or mRNA
or other compounds to be injected are aspirated into the injection pipette by upward movement
of the plunger and then pushed into either the nucleus or the cytoplasm by downward movement
of the plunger.
The provided microscope is mandatory for the alignment process. Place the microscope
in a position onto the x/y table which allows to focus on the alignment device plugged
in well H12 of the well plate. Please read chapter “Alignment” for detailed information.