Multichannel Systems Roboinject Manual - Manual (Page 27)

Roboinject
23
Example on the picture: The bottom of the well is value zero, the reference value. If you define,
for example an impalement position of 650 μm, the injector mount will move down until its
position is 650 μm above the bottom of the well. If you additionally define an injection position
of 550 μm, the injector mount will move down again until its position is 550 μm above the bottom
of the well.
If you define, for example an impalement position of 300 μm, the injector mount will move down
until its position is 300 μm above the bottom of the well. If you additionally define an injection
position of 550 μm, the injector mount will move up until its position is 550 μm above the bottom
of the well.
The “Wait between Movements” in ms as well as the "Pauses before and after Injection"
can be adjusted to user preferences.
“Injector”
The injection interval is terminated by the time frame before injection "Pause before
Injection", and the time frame after injection "Pause after Injection", both measured in ms.
The default values for the wait times before and after injection (500 ms and 2000 ms, respectively)
have been tested with oocytes and RNA injections.
You have to define the injection “Volume” in nl, and the “Velocity” in nl/s from the up-down
controls. The respective default values (50 nl and 70 nl/s) have been tested with oocytes and
should work in most cases.