Multichannel Systems Roboinject Manual - Manual (Page 49)

Roboinject
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Defining the Number of Injections into a single Oocyte
In some experiments it is necessary to inject more than one compound into a single oocyte.
The Roboinject program gives the possibility for up to four injections into the same oocyte.
To define the well for several injections, please click onto the tube icon first, and after that
onto the well. The color of the well will immediately change into the symbolic color of the
sample of the selected tube.
Select a second tube icon, and click onto the same well again. One half of the color of the well
will immediately change into the color of the second sample.
Select a third tube icon, and click onto the same well again. One third of the color of the well
will immediately change into the color of the third sample. Select a fourth tube icon, and click
onto the same well again. One quarter of the color of the well will immediately change into
the color of the fourth sample.
Click "Clear Plate" to set back all wells to the origin, non-colored status. The deselected
(crossed out) wells will remain. After successful injection the wells (or sections of the well)
will be marked with an arrow.