Leica Biosystems ST5020 - Manual (Page 64)

64 Instructions for Use, V1.9, RevD - 12/2012
Setting the dips
• Press DIP to switch from yes (= Dips activated) to no and vice versa.
• Duration and speed of one complete up and down movement (dip) are
selected in the MOVEMENT menu. The parameters selected there will
be applied to all programs. Increasing numbers of dips reduce instru-
ment throughput.
For exact steps the following applies:
If the immersion time of an exact step is shorter than the time to
do the set number of dips, only the number of dips that fit into
the immersion time will be done.
Defining exact steps
As there is potential for timing clashes to occur when multiple
racks are present in the instrument, steps which require pre-
cise timing are designated as ‘exact’ in the program.
• Press EXACT to alter between yes (=exact) and no (not exact).
• Immersion times at exact steps are achieved to within ± 1 second.
Immersion time at non-exact steps is at least as long as defined, most
of the time however, non-exact steps take longer than defined, as
racks due for non-exact steps receive attention only as the transfer
arm becomes available after an exact step.
• Define ‘exact’ steps only when really needed. The more exact steps,
the slower and less flexible the instrument (instrument throughput de-
creases).
If you interrupt the staining with PAUSE, take care not to do so
while an exact step is in progress. Otherwise, the program will
be stopped and the duration of the step will be extended by the
duration of the pause. This can the cause errors during stain-
ing.
If PAUSE buttun has been pressd druring an exact step is in
process, a warnig message will be appear in the dialog box
5. Operation
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