Leica Biosystems ST5020 - Manual (Page 65)

65 Leica ST5020
Completing the program
• Once the parameters EXACT and DIP have been selected, the defini-
tion of your step is complete.
• Press INSERT ROW to create a new row for the next program step and
once again define all step parameters as described above.
• Keep creating program steps until your program is complete.
• Program steps whose definition is not correct (duplicate station
assignment, incorrect reagent,...) are highlighted in red in the
REAGENT column. Such steps must be corrected accordingly.
• A program will not be ready to start until all details have been defined.
The last program step
Three different types of “last steps“ can be defined:
1. Last step = unload station.
Go to CHOOSE STATION and select EXIT as last station.
2. Last step = transfer station to Cover Slipper (optional).
go to CHOOSE STATION and select CV as last station.
The REAGENT column from the reagent list now allows selecting
between CV program 1 to CV program 4.
The transferred rack is automatically processed with the selected pro-
gram (1 ... 4) in the CV5030.
3. Last step = any station other than EXIT or CV.
Programs do not necessarily have to finish in the unload station, as a
particular staining protocol might e.g. have to end with a specific re-
agent not contained in the last station.
Be sure to define the DURATION of the last step. Otherwise it
will not be carried out (default duration 00:00 will be assigned).
Otherwise, the program cannot be started.
5. Operation