8 staining, 1 loading the racks, Staining – Leica Biosystems ST5020 User Manual

Page 69: Loading the racks, Operation

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69

Leica ST5020

5.

Operation

5.8 Staining

Prior to starting a staining program, thefollo-
wing preparatory steps must be taken:

Optimize all USER INTERFACE parameters.

Programs needed for staining must be
‘ready-to-start’.

Stations have to be configured and filled
according to configuration.

Racks needed for staining must be equipped
with a clip and filled with the slides to be
processed.
Place the clips (1) onto the rack with the
transponder (2) facing outwards.

Make sure the instrument “knows“ all clips
needed for staining.

Fig. 66

Racks may only be loaded via the load
stations (front right of the instrument).

Open the load drawer and insert the racks
as shown in

Fig. 66

in one of the load

stations with the clips facing outwards
(towards the user).

Each rack must bear a clip corresponding to
the program to be run.

Close the drawer completely.

For the clips to be recognized
correctly, the load drawer must be
opened for loading and
subsequently be closed again. Do
not insert the racks just opening the
lid!

2

1

5.8.1 Loading the racks

Caution with sequences which
involve an oven at the initial step.
In this case the loading unit, out of
which the specimen holder is
removed via the transport arm, must
NOT be filled with an inflammable
reagent (e.g. xylene).
As the oven temperature is up to
80 °C (176 °F), the reagent may ignite
and cause damage to the device
and to the samples.

For the same reason, steps
conducted INSIDE an oven must
never be carried out from a reagent
station with inflammable reagents.

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