Gilson 402 - Manual (Page 111)

Appendix C-3
Technical Data Appendix
C
Hydraulics
Syringes
Performance Specifications
The following values are obtained using a defined
protocol. If this protocol is followed, the obtained
values must be inferior or equal to the listed figures.
Syringe capacity (µL) 100 250 500 1000 5000 10000 25000
Maximum Flow (mL/min) 4 10 20 40 120 120 120
Minimum Specified Volume (µL) 10 25 50 100 500 1000 2500
Nominal Volume (µL) 100 250 500 1000 5000 10000 25000
Protocol
Configuration: 1 mL tubing for volumes up to 1 mL,
tapered transfer-tubing of 2 mL (standard accessory)
for volumes up to 2 mL, 5 mL or 10 mL tapered
transfer tubings are used for volumes up to 10 mL.
Transfer of de-ionized water using the Pipette Task,
aspiration of liquid from a vial and delivery at the
same flow rate into another vial containing water.
Syringe Volume Systematic error Random error
capacity (µL) pipetted (µL) % µL % µL
10 1.8 0.18 0.8 0.08
100 50 1.5 0.75 0.25 0.125
100 1 1 0.15 0.15
20 1.5 0.38 0.8 0.2
250 125 1.0 1.25 0.25 0.32
250 0.8 2 0.15 0.38
50 1.5 0.75 0.50 0.25
500 250 1.0 2.5 0.25 0.63
500 0.8 4 0.15 0.75
100 1.5 1.5 0.50 0.5
1000 500 0.8 4 0.25 1.3
1000 0.6 6 0.15 1.5
500 1.2 6 0.5 2.5
5000 2500 0.8 20 0.25 6.3
5000 0.6 30 0.15 7.5
1000 1.2 12 0.5 5
10000 5000 0.8 40 0.25 12.5
10000 0.5 50 0.15 15
2000 1.0 25 0.50 5
25000 12500* 0.8 100 0.25 31.3
25000* 0.5 125 0.15 37.5
* Dispensing mode only.
The values are obtained by
pipetting de-ionized water
(grade 3, ISO3696) at 21 °C ± 0.5 °C,
relative humidity > 50 % and
athmospheric pressure 1013 MPa.