Gilson 402 - Manual (Page 84)

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Operation - Advanced Use
5
Gilson D200 tips are used for volumes from 1 µL to
78 µL (with a 100 µL syringe, minimum recommended
air-push-volume is 20 µL). Gilson D1000 tips are used
for volumes from 50 µL to 900 µL (minimum
recommended air-push-volume is 100 µL).
The liquid is ejected with an Air push volume of at
least 20 µL when using a Gilson D200 tip and 100 µL
when using a Gilson D1000 tip. An automatic
software correction of 2% of the piston stroke
is made to improve accuracy with Gilson D200 and
D1000 tips. For example, when using a Gilson D200
tip, if an Air push volume of 20 µL is chosen, the
maximum sample volume will be 78 µL; this volume
will be handled with the 100 µL syringe. For the dual
syringe model, the left syringe operates if the
volume exceeds 78 µL; for the single syringe model,
an error message is displayed.
A disposable tip should not be fitted, when priming
the 402 in ‘Air Mode’. Droplets of liquid adhering
the tubing after purging must be wiped from the
end, before fitting a tip.
To minimize carry-over, dilution can be performed
with one or more tips. If one tip is used, you use the
Dilute task where the diluent and the sample are
separated by an air gap (volume defined in the
Configuration menu). When several tips are used,
you use multiple runs of the Dispense task to
transfer the liquids, changing the tips where appro-
priate: one to aspirate and dispense the diluent, one
to aspirate and dispense each sample.
Liquid Mode
You can use this mode in the same way as the
standard tip with tapered tubing. However, it is
recommended not to use an air gap, or at least to use
it in a way that keeps the air gap in the disposable-
tip. The use of Gilson D200 tips does not adversely
affect the performance of the 402.
The Gilson
D200 tip is installed to protect the end
of the probe against corrosive chemicals, and by
changing the tips within a task.
A disposable tip should be fitted, when priming the
402 in ‘Liquid Mode’. The following flow rates are
suggested when priming: small syringes up to 10 mL/
min, larger syringes at least 80 mL/min.
Additional Information