Operation - advanced use, Direct manipulations (man) – Gilson 402 User Manual

Page 62

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Operation - Advanced Use

5

To unlock this file you must key in the code that

was used to lock the file. You may not edit a locked

file, so the Edit soft-key is replaced by the View

soft-key, which allows you to browse through the

contents of that file, without making any changes.
However, you are permitted to copy a locked file.

After copying the original file remains locked but the

copied file is unlocked automatically by the software.

Direct Manipulations (MAN)

You can either perform one of three direct manipu-

lations (Prime, Measure, Titrate) by pressing the

corresponding soft-key. Press the Man soft-key, the

Action Screen appears.

Prime

You should prime the 402 before each run, to ensure

that all fluid pathways contain fresh solvent.
Press Prime, Initialization Screen 2 appears.
Put the probe-end of the transfer-tubing into a

suitable waste container, then press OK.
Priming starts immediately and the message

‘Priming ...’ appears on the screen; during which

time solvent is expelled at the probe-end of the

transfer-tubing.
Press STOP, or the End soft-key, when you are

satisfied that all fluid pathways have been primed

with fresh solvent and that there are no bubbles in

the tubing.

Pressing End

When you press End, the motion of the syringe(s)

ceases and ‘Homing ...’ appears on the screen until

the syringe(s) have returned to their uppermost

positions.

Pressing STOP

You can press STOP at any time, however, this key

is more for use if a problem arises (such as a leak).
When you press STOP, the motion of the syringe(s)

ceases and the Abort Screen appears. Press Proceed,

when you want to continue the prime from the point

at which it was stopped (for example, to refill the

solvent reservoir).

Software

Locked File Screen

Action Screen

Initialization Screen

Abort Screen

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