Gilson 402 - Manual (Page 69)

5-19
Operation - Advanced Use
5
Press Next, then key in the sample volume, press
ENTER.
Depending on the mode,
you see one of three
screens after pressing
Next.
Key in the value, press ENTER.
Optionally, for any
mode, you can display
the variables calculated
by the software by pressing Data.
Optionally, you can modify the flow rates; press Flow,
For any mode, key in a
value for Asp flow,
press ENTER, press
Next, key in a value for
Disp flow, press ENTER.
Optionally, for any mode, you can supply up to 12
reagents, press Reag, key in number of reagents,
press ENTER.
Press Next, then for each reagent, key in volume
press ENTER.
After the data are entered the Reag key changes to
REAG.
Press ESC until you see this screen (three times if
you have added reagents);
Press ESC again to reveal the Save Screen; select
Yes, the Method is then saved, after which you see
the Run Screen.
Dilute Serially
You use Dilute Serially to dilute
samples, with diluent from the
reservoir or via the probe. You
also have the option to mix after
dilution, but the addition of
reagents is restricted to Dilute
Normal and Dilute Unknown.
There are 3 modes of entering
the parameters, in each mode
you supply Sample Volume
(Vs) plus one of the following
variables:
Dilution Ratio (Rd), Diluent Volume (Vd), and
Total Volume (Vt). The software calculates the
remaining variables. In any mode, pressing the
Data soft-key gives the calculated values for the
other two parameters.
Software
1 4 7 10
dilution 1 dilution 3 dilution 2
2
3
result
1
5
6
result
1
8
9
result