Gilson 402 - Manual (Page 66)

5-16
Operation - Advanced Use
5
Keys
The use of the soft-keys is as follows:
- Prev is used to go to the previous task (or screen
within a task),
- Next is used to go to the next task (or screen within
a task),
- Add is used to add a task to the Method (after the
task displayed),
- Param is used to access the parameters for the
current task,
- Ins is used to insert a task (before the task displayed),
- Del is used to remove the task currently displayed.
Parameters
The following list summarizes the parameters used
by the tasks; it gives the maximum and minimum
values for each parameter. The maximum values
are mostly unambiguous. However, in the case of
volumes aspirated via the probe, the total volume of
fluids (air and liquids) aspirated by a task can never
exceed the internal volume of the transfer tubing.
The maximum values listed for volumes aspirated
via the probe are guidelines that you should follow
when considering parameter values to enter. In some
cases you may be able to key in a larger value but
this will provoke a self explanatory error message,
immediately after entry. Even then, depending on
the task, you may key in a series of parameter values,
for which the software will then calculate the total
volume. If the transfer-tubing volume is exceeded,
you will be advised of the required configuration.
For example, you have configured you 402 with the
standard 2 mL transfer tubing. In a task, where you
can aspirate more than one liquid (e.g. sample and
reagents), the total volume of all fluids adds up to
2110 µL. The software issues a warning message
(for example, ‘Tubing too small’). You press the OK
key to acknowledge the message, after which will you
see ‘Parameters Incompatible!’ on the screen. At this
point you can either press Quit and reconfigure the
402 or press OK and renter one or more of the
volumes entered in the task.
When you configure your 402 for use with dispos-
able tips, the ‘Extra volume’ parameter is replaced
automatically by ‘Air-push volume’ in the Dilute,
Distribute and Pipette tasks, corresponding to the
special requirements of this type of configuration;
refer to pages 5-33, 5-34 for additional information.
Software