Operation - advanced use, Additional information, Flow rates - dual-syringe model – Gilson 402 User Manual
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Operation - Advanced Use
5
Additional Information
Flow Rates - Dual-syringe Model
Flow rates are the values entered in Flow menus
(Asp Flow or Disp Flow).
• It is not possible to enter a flow rate greater than
the maximum without entering an air gap for the
left-hand syringe.
• If a flow rate (aspirate or dispense) is greater than
the maximum for the right-hand syringe, the left-
hand syringe aspirates and dispenses at the
selected flow rate. The right-hand syringe
aspirates and dispenses as its maximum flow
rate.
• If a flow rate is equal to or smaller than the
maximum for the right-hand syringe, both
syringes aspirate and dispense at the selected
flow rate.
Example
When using the 2 mL tapered transfer-tubing,
a 5 mL syringe at the left-hand side and a 500 µL
syringe at the right-hand side, the default aspirate
flow rates are respectively 100 mL/min and
20 mL/min. The dispense flow rate, accessible by
pressing Deflt soft-key, is 120. The implicit flow
rate of the small syringe is 20 mL/min.
If the value of the flow rate is between 120 and 20, the
flow rate of the left-hand syringe takes this value
and the flow rate of the right-hand syringe is 20. To
reduce to the flow rate of the right-hand syringe, it
is necessary to enter values below 20 mL/min.
Maximum Dispense Flow Rate
(No Air Gaps)
Syringe Volume (µL)
Tubing Type
100
250
500
1000
5000
10000
25000
1 mL
4
10
20
40
120
120
120
2 mL
4
10
20
40
120
120
120
5 mL
4
10
20
40
120
120
120
10 mL
4
10
20
40
120
120
120
Micro Tips (liquid)
4
10
20
40
120
120
120
Micro Tips (air)
4
10
20
40
120
120
120
Large Tips (air)
4
10
20
40
120
120
120
Recommended Flow Rate with
an Air Gap (10 µL)
Syringe size
Flow rate
(mL/min)
100 µL
4
250 µL
10
500 µL
20
1 mL
30
5 mL
Asp: 30 - Disp: 50
10 mL
Asp: 30 - Disp: 50
25 mL
Asp: 30 - Disp: 50