Gilson 402 - Manual (Page 63)

5-13
Operation - Advanced Use
5
Press Abort, when you want to stop priming;
Initialization Screen 2 reappears. Ensure that the
probe-end of the transfer-tubing is in a suitable
waste container, then press OK. ‘Homing ...’
appears on the screen until the syringe(s) have
returned to their uppermost (home) positions,
during which time any liquid contained in the
transfer-tubing is expelled from the probe-end.
After this the Action Screen reappears.
Press ESC to return to the start-up screen for the
current file.
Whenever you see ‘Homing’ on the screen, the
syringe piston(s) are returned to their uppermost
position(s). As a result, some of the liquid con-
tained in the transfer-tubing will be expelled
from the probe-end. You must ensure that the
probe end of transfer-tubing is already in a
suitable waste container.
Measure
You can measure a volume (e.g. supernatant after
centrifugation).
• Press Measure, the Measure Screen appears.
For a Dual-syringe 402, press the Chge soft-key to
select LEFT or RIGHT. You are advised to select
the left-hand syringe if the volume to be measured
seems to be significantly larger than the capacity
of the right-hand syringe.
The maximum volume that you can measure is
limited to the volume of the aspirating syringe or
the transfer tubing size, whichever is the smaller.
• Press ENTER, the Flow Rate Screen appears.
Key in the flow rate at which you want the
volume measurement to take place, and then
press ENTER. The default flow rate is half the
maximum possible for the combination of syringe
and transfer tubing in use (refer to page 5-31).
Press Run, then follow the instructions given on the
screen; you press the command button on the probe
to aspirate, when prompted to do so by the com-
mand light on the probe:
- a steady light means you are to aspirate (or
dispense) liquid,
- a flashing light means you are to aspirate air via
the probe.
Software
Measure Screen
Flow Rate Screen